BALANCING TIME AND COST IN RESOURCE-CONSTRAINED PROJECT SCHEDULING USING META-HEURISTIC APPROACH


Fine tuning of spatial arrangement of enzymes in a PCNA-mediated multienzyme complex using a rigid poly-L-proline linker.

Inspired by natural multienzyme complexes, many types of artificial multienzyme Incense complexes have recently been constructed.We previously constructed a self-assembled complex of a bacterial cytochrome P450 and its ferredoxin and ferredoxin reductase partners using heterotrimerization of proliferating cell nuclear antigen (PCNA) from Sulfolobus

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